Coding

Part:BBa_K2730010:Design

Designed by: Xinyue Ma   Group: iGEM18_Tongji_China   (2018-10-08)


T3SS-neoantigen EX0301A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 429


Design Notes

The neo-antigen part added in MSC of T3SS part is selected as it has stronger binding potential to possible antigen.



Source

The neo-antigen part is from SNVs (single nucleotide variations) of Homo Sapiens selected by bioinformatic methods which occur during the development of colorectal cancer cell. The T3SS part comes from natural Pseudomonas aeruginosa.


References